Genomic DNA from forensic samples User manual NucleoSpin DNA Trace June 2014 Rev 07 MACHEREY NAGEL www mn net com Genomic DNA from forensic samples Protocol at a glance Rev 07 Funnel NucleoSpin DNA Trace 1 Lyse sample 4 8 mL FLB 50 uL Proteinase K 56 C 1h 2 Clarify sample e 2 5 000 x g 10 min 3 Adjust DNA binding sonditians 3 5 mL ethanol Vortex 4 Bind DNA am il Load sample y 3 000 x g e
www mn net com Genomic DNA from blood User manual NucleoSpin Dx Blood ce IVD REF aal Cli MACHEREY NAGEL In Vitro Diagnostic Medical Device 740899 50 740899 250 MACHEREY NAGEL GmbH amp Co KG D 52355 D ren Tel 49 0 2421 969 0 June 2014 Rev 03 Genomic DNA from blood Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 6 1 3 About this use
Genomic DNA from plant User manual NucleoSpin 96 Plant II NucleoSpin 96 Plant II Core Kit May 2014 Rev 03 MACHEREY NAGEL MN www mn net com Genomic DNA from plant Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use of the NucleoSpin 96 Plant II Core Kit 8 2 5 Automated
Plasmid DNA purification User manual NucleoSpin 8 Plasmid NucleoSpin 8 Plasmid Core Kit April 2014 Rev 03 MACHEREY NAGEL MN www mn net com Plasmid DNA purification Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 8 Plasmid Core Kit 9 2 5 Automat
PCR clean up User manual NucleoSpin 96 PCR Clean up NucleoSpin 96 PCR Clean up Core Kit April 2014 Rev 04 MACHEREY NAGEL www mn net com PCR clean up Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 96 PCR Clean up Core Kit 8 2 5 Automated process
Genomic DNA from food User manual NucleoSpin 96 Food April 2014 Rev 04 MACHEREY NAGEL www mn net com Genomic DNA from food Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 5 2 1 The basic principle 5 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Automated processing on robotic platforms 7 2 5 Sample storage and homogenization 8 2 6 Elution procedures 8 3 Storage condit
Genomic DNA from forensic samples User manual NucleoSpin DNA Trace March 2012 Rev 05 MACHEREY NAGEL MN Genomic DNA from forensic samples Protocol at a glance Rev 05 Funnel NucleoSpin DNA Trace 1 Lyse sample 4 8 mL FLB 50 uL Proteinase K 56 C th 2 Clarify sample es 2 5 000 x g 10 min 3 Adjust DNA binding conditions 3 5 mL ethanol Vortex 4 Bind DNA baum i Load sample 7 3 000 x g C 3 min 5 Wash si
PCR clean up User manual NucleoSpin 8 PCR Clean up NucleoSpin 8 PCR Clean up Core Kit April 2014 Rev 03 MACHEREY NAGEL www mn net com PCR clean up Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 8 PCR Clean up Core Kit 8 2 5 Automated processing
Genomic DNA clean up User manual NucleoSpin gDNA Clean up July 2014 Rev 02 MACHEREY NAGEL www mn net com gDNA clean up Protocol at a glance Rev 02 1 Adjust DNA binding conditions NucleoSpin gDNA Clean up 150 uL sample 450 uL DB Vortex 5 s For smaller sample volumes adjust to 150 uL with water for larger sample volumes increase binding buffer proportionally 2 Bind DNA Load sample on NucleoSpin gDNA Clean up Column 11
Genomic DNA from Plant User Manual NucleoSpin Plant II NucleoSpin Plant Il Midi NucleoSpin Plant Il Maxi This product is distributed by Clontech Laboratories Inc A Takara Bio Company 1290Terra Bella Ave Mountain View CA 84043 www clontech com AEG Website for more details 5 For ordering information j clickchere ae 1 800 662 2566 PENARI orders clontech com PR113798 For technical support 1 800 662 2566 Clontec
Genomic DNA from blood User manual NucleoSpin 8 Blood NucleoSpin 8 Blood Core Kit April 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from blood Table of contents 1 Components 1 1 Kit contents 1 2 Reagent to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use ofthe NucleoSpin 8 Blood CoreKit 8 2 5 Automated processing on
Genomic DNA from forensic samples User manual NucleoSpin 96 Trace April 2014 Rev 03 MACHEREY NAGEL www mn net com Genomic DNA from forensic samples Table of contents 1 Components 1 1 Kit contents 1 2 Reagent to be supplied by user 4 2 Product description 5 2 1 The basic principle 5 2 2 Kit specifications 5 2 3 Required hardware 6 3 Storage conditions and preparation of working solutions 8 4 Safety instructions 9 5 Protocols 11 5 1 Nucle
Plasmid DNA purification User manual NucleoSpin 96 Plasmid NucleoSpin 96 Plasmid Core Kit April 2014 Rev 03 MACHEREY NAGEL MN www mn net com Plasmid DNA purification Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use of the NucleoSpin 96 Plasmid Core Kit 8
Viral nucleic acid isolation User manual NucleoSpin Dx Virus A M In Vitro Diagnostic Medical Device S 740895 50 J m T MACHEREY NAGEL GmbH amp Co KG D 52355 D ren Tel 49 0 2421 969 0 JE July 2014 Rev 04 MACHEREY NAGEL www mn net com MN Viral nucleic acid isolation Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 6 1
Genomic DNA from microoganisms User manual NucleoSpin Microbial DNA August 2015 Rev 01 MACHEREY NAGEL MN www mn net com Genomic DNA from microorganisms Protocol at a glance Rev 01 NucleoSpin Microbial DNA lt 40 mg microbial pellet wet weight 1 Prepare sample 100 uL BE Transfer sample in NucleoSpin Bead Tube Type B 40 uL Buffer MG 2 Sample lysis eS 10 uL Liquid Proteinase K Agitate on a swing mill or similar device 4 12 min 11
Genomic DNA from tissue User manual NucleoSpin 96 Tissue NucleoSpin 96 Tissue Core Kit May 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from tissue Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Accessories supplied for use of the NucleoSpin 96 Tissue Core Kit 8 2 5 Automated processi
RNA from blood User manual NucleoSpin RNA Blood NucleoSpin RNA Blood Midi May 2014 Rev 03 MACHEREY NAGEL MN www mn net com RNA from blood Protocol at a glance Rev 03 NucleoSpin NucleoSpin NucleoSpin RNA Blood RNA Blood RNA Blood Midi 200 uL blood 400 pL blood 1 3 mL blood 1 Lyse blood 200 pL blood 400 uL blood 1 3 mL blood 200 uL DL 400 uL DL 1 8 mL DL Mix Mix Mix 5
Total RNA isolation from FFPE Samples User manual NucleoSpin totalRNA FFPE NucleoSpin totalRNA FFPE XS July 2015 Rev 04 MACHEREY NAGEL MN www mn net com Total RNA from FFPE samples Protocol at a glance Rev 04 page 1 1 Sample preparation NucleoSpin totalRNA FFPE Insert FFPE section s in a microcentrifuge tube NucleoSpin totalRNA FFPE XS Insert FFPE section s in a microcentrifuge tube 2 Deparaffinize sample 1 mL Paraffin Di
Genomic DNA from soil User manual NucleoSpin 96 Soil July 2014 Rev 02 MACHEREY NAGEL www mn net com Genomic DNA from soil Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 5 1 3 About this user manual 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Automated processing on robotic platforms 7 2 4 Relevance of humic substances as PCR inhibito
DNA isolation from FFPE samples User manual NucleoSpin DNA FFPE XS June 2014 Rev 02 MACHEREY NAGEL www mn net com DNA Isolation from FFPE Samples Protocol at a glance Rev 02 NucleoSpin DNA FFPE XS Protocol 5 1 DNA isolation with Paraffin Dissolver Protocol 5 2 DNA isolation with xylene Sample preparation For appropriate sample For appropriate sample quantity see section 2 4 quantity see section 2 4 1