Genomic DNA from microoganisms User manual NucleoSpin Microbial DNA August 2015 Rev 01 MACHEREY NAGEL MN www mn net com Genomic DNA from microorganisms Protocol at a glance Rev 01 NucleoSpin Microbial DNA lt 40 mg microbial pellet wet weight 1 Prepare sample 100 uL BE Transfer sample in NucleoSpin Bead Tube Type B 40 uL Buffer MG 2 Sample lysis eS 10 uL Liquid Proteinase K Agitate on a swing mill or similar device 4 12 min 11
www mn net com Genomic DNA from blood User manual NucleoSpin Dx Blood ce IVD REF aal Cli MACHEREY NAGEL In Vitro Diagnostic Medical Device 740899 50 740899 250 MACHEREY NAGEL GmbH amp Co KG D 52355 D ren Tel 49 0 2421 969 0 June 2014 Rev 03 Genomic DNA from blood Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 6 1 3 About this use
Genomic DNA from food User manual NucleoSpin Food July 2014 Rev 11 MACHEREY NAGEL www mn net com Genomic DNA from food Protocol at a glance Rev 11 NucleoSpin Food 1 Homogenize sample Homogenize 200 mg material 2 Lyse cells 550 uL CF 65 C 10 uL Proteinase K 65 C 30 min cS gt 10 000 x g 10 min Take clear supernatant 1 vol and continue with step 3 3 Adjust DNA binding conditions 1 vol C4 1 vol
NucleoSpin Extract II Kits User Manual Cat Nos 636971 636972 636973 PT3814 1 PR48598 Published 10 September 2004 NucleoSpin Extract ll Kits User Manual Table of Contents I Kit Contents 3 ll Product Description 4 A The Basic Principle 4 B About this User Manual 4 C Kit Specifications 4 D Elution Procedures 5 lll Storage Conditions and Preparation of Working Solutions 8 IV Safety Instructions Risk and Safety Phrases 9 V Protocol for DNA Ext
Genomic DNA from soil User manual NucleoSpin 96 Soil July 2014 Rev 02 MACHEREY NAGEL www mn net com Genomic DNA from soil Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 5 1 3 About this user manual 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Automated processing on robotic platforms 7 2 4 Relevance of humic substances as PCR inhibito
RNA isolation User manual NucleoSpin RNA XS September 2015 Rev 09 MACHEREY NAGEL www mn net com SUN RNA isolation Protocol at a glance Rev 09 XS NucleoSpin RNA XS Use up to 10 cultured cells 1 Supply sample or 5 mg tissue samples TT 100 pL RA1 2 Lyse and 2 uL TCEP homogenize cells y Mix 5 uL Carrier RNA 3 Add Carrier RNA working solution Mix 4 Filtrate lys
Genomic DNA from Plant User Manual NucleoSpin Plant II NucleoSpin Plant Il Midi NucleoSpin Plant Il Maxi This product is distributed by Clontech Laboratories Inc A Takara Bio Company 1290Terra Bella Ave Mountain View CA 84043 www clontech com AEG Website for more details 5 For ordering information j clickchere ae 1 800 662 2566 PENARI orders clontech com PR113798 For technical support 1 800 662 2566 Clontec
RNA clean up User manual NucleoSpin RNA Clean up May 2014 Rev 05 MACHEREY NAGEL www mn net com RNA clean up Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 5 1 3 About this user manual 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Handling preparation and storage of starting materials 7 2 4 Elution procedures 8 3 Storage conditions and
PCR clean up User manual NucleoSpin 96 PCR Clean up NucleoSpin 96 PCR Clean up Core Kit April 2014 Rev 04 MACHEREY NAGEL www mn net com PCR clean up Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 96 PCR Clean up Core Kit 8 2 5 Automated process
Viral nucleic acid isolation User manual NucleoSpin Dx Virus A M In Vitro Diagnostic Medical Device S 740895 50 J m T MACHEREY NAGEL GmbH amp Co KG D 52355 D ren Tel 49 0 2421 969 0 JE July 2014 Rev 04 MACHEREY NAGEL www mn net com MN Viral nucleic acid isolation Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 6 1
RNA and protein purification User manual NucleoSpin RNA Protein May 2014 Rev 09 MACHEREY NAGEL www mn net com RNA Purification Total RNA and protein isolation Protocol at a glance Rev 09 NucleoSpin RNA Protein 1 Homogenization e a0 tA of sample g 2 Cell lysis 350 uL RP1 3 5 uL B mercapto ethanol 3 Filtration of lysate 11 000 x g E 5 1 min el ag 350 pL ethanol 70 conditions 5 Bind RNA 11 000 x g 30s
Genomic DNA from tissue User manual NucleoSpin 8 Tissue NucleoSpin 8 Tissue Core Kit May 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from tissue Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Accessories supplied for use of the NucleoSpin 8 Tissue Core Kit 8 2 5 Automated processing
Plasmid DNA purification User manual NucleoSpin 8 Plasmid NucleoSpin 8 Plasmid Core Kit April 2014 Rev 03 MACHEREY NAGEL MN www mn net com Plasmid DNA purification Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 8 Plasmid Core Kit 9 2 5 Automat
Total RNA isolation User manual NucleoSpin 8 RNA NucleoSpin 8 RNA Core Kit April 2014 Rev 05 MACHEREY NAGEL MN www mn net com Total RNA isolation Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Yield and quality of total RNA 6 2 4 Required hardware 8 2 5 Recommended accessories for use of the NucleoSpin 8 RNA Core Kit
Genomic DNA from plant User manual NucleoSpin 96 Plant II NucleoSpin 96 Plant II Core Kit May 2014 Rev 03 MACHEREY NAGEL MN www mn net com Genomic DNA from plant Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use of the NucleoSpin 96 Plant II Core Kit 8 2 5 Automated
RNA isolation from plant User manual NucleoSpin RNA Plant May 2014 Rev 09 MACHEREY NAGEL www mn net com RNA isolation from plant Protocol at a glance Rev 09 NucleoSpin RNA Plant 1 Homogenize sample c 100 mg Lyse cells 350 uL RA1 3 5 uL B mercaptoethanol or 350 uL RAP 3 5 uL B mercaptoethanol Mix Filtrate lysate 11 000 x g 1 min Adjust RNA binding conditions 350 uL 70 ethanol Mix Bind R
Genomic DNA from tissue User manual NucleoSpin 96 Tissue NucleoSpin 96 Tissue Core Kit May 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from tissue Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Accessories supplied for use of the NucleoSpin 96 Tissue Core Kit 8 2 5 Automated processi
Genomic DNA from blood User manual NucleoSpin 8 Blood NucleoSpin 8 Blood Core Kit April 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from blood Table of contents 1 Components 1 1 Kit contents 1 2 Reagent to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use ofthe NucleoSpin 8 Blood CoreKit 8 2 5 Automated processing on
DNA isolation from FFPE samples User manual NucleoSpin DNA FFPE XS June 2014 Rev 02 MACHEREY NAGEL www mn net com DNA Isolation from FFPE Samples Protocol at a glance Rev 02 NucleoSpin DNA FFPE XS Protocol 5 1 DNA isolation with Paraffin Dissolver Protocol 5 2 DNA isolation with xylene Sample preparation For appropriate sample For appropriate sample quantity see section 2 4 quantity see section 2 4 1
RNA and DNA purification User manual NucleoSpin RNA DNA Buffer Set May 2014 Rev 08 MACHEREY NAGEL MN www mn net com RNA and DNA purification Protocol at a glance Rev 08 1 Homogenize NucleoSpin RNA miRNA RNA Blood RNA Plant NucleoSpin RNA Protein NucleoSpin RNA XS sample So Sample Sample 2 Lyse cells 350 uL RA1 RAP or RP1 100 uL RA1 y 3 5 uL reducing agent 2 uL TCEP Mix Mix 5 uL Carrier RNA 3 Filtrate lysate cS 11 0