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1. NucleoSpin® Food - MACHEREY

Genomic DNA from food User manual NucleoSpin Food July 2014 Rev 11 MACHEREY NAGEL www mn net com Genomic DNA from food Protocol at a glance Rev 11 NucleoSpin Food 1 Homogenize sample Homogenize 200 mg material 2 Lyse cells 550 uL CF 65 C 10 uL Proteinase K 65 C 30 min cS gt 10 000 x g 10 min Take clear supernatant 1 vol and continue with step 3 3 Adjust DNA binding conditions 1 vol C4 1 vol
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1. NucleoSpin® Microbial DNA - MACHEREY

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2. NucleoSpin® Dx Blood - MACHEREY

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3. NucleoSpin® Food - MACHEREY

Genomic DNA from food User manual NucleoSpin Food July 2014 Rev 11 MACHEREY NAGEL www mn net com Genomic DNA from food Protocol at a glance Rev 11 NucleoSpin Food 1 Homogenize sample Homogenize 200 mg material 2 Lyse cells 550 uL CF 65 C 10 uL Proteinase K 65 C 30 min cS gt 10 000 x g 10 min Take clear supernatant 1 vol and continue with step 3 3 Adjust DNA binding conditions 1 vol C4 1 vol
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4. NucleoSpin® Extract II Kits

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5. NucleoSpin® 96 Soil - MACHEREY

Genomic DNA from soil User manual NucleoSpin 96 Soil July 2014 Rev 02 MACHEREY NAGEL www mn net com Genomic DNA from soil Table of contents 1 Components 4 1 1 Kit contents 4 1 2 Reagents consumables and equipment to be supplied by user 5 1 3 About this user manual 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Automated processing on robotic platforms 7 2 4 Relevance of humic substances as PCR inhibito
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6. NucleoSpin® RNA XS - MACHEREY

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8. NucleoSpin® RNA Clean-up - MACHEREY

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9. NucleoSpin® 96 PCR Clean-up - MACHEREY

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10. NucleoSpin® Dx Virus - MACHEREY

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11. NucleoSpin® RNA/Protein - MACHEREY

RNA and protein purification User manual NucleoSpin RNA Protein May 2014 Rev 09 MACHEREY NAGEL www mn net com RNA Purification Total RNA and protein isolation Protocol at a glance Rev 09 NucleoSpin RNA Protein 1 Homogenization e a0 tA of sample g 2 Cell lysis 350 uL RP1 3 5 uL B mercapto ethanol 3 Filtration of lysate 11 000 x g E 5 1 min el ag 350 pL ethanol 70 conditions 5 Bind RNA 11 000 x g 30s
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12. NucleoSpin® 8 Tissue - MACHEREY

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13. NucleoSpin® 8 Plasmid (Core Kit) - MACHEREY

Plasmid DNA purification User manual NucleoSpin 8 Plasmid NucleoSpin 8 Plasmid Core Kit April 2014 Rev 03 MACHEREY NAGEL MN www mn net com Plasmid DNA purification Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 8 2 4 Recommended accessories for use of the NucleoSpin 8 Plasmid Core Kit 9 2 5 Automat
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14. NucleoSpin® 8 RNA - MACHEREY

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15. NucleoSpin® 96 Plant II (Core Kit) - MACHEREY

Genomic DNA from plant User manual NucleoSpin 96 Plant II NucleoSpin 96 Plant II Core Kit May 2014 Rev 03 MACHEREY NAGEL MN www mn net com Genomic DNA from plant Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Recommended accessories for use of the NucleoSpin 96 Plant II Core Kit 8 2 5 Automated
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16. NucleoSpin® RNA Plant - MACHEREY

RNA isolation from plant User manual NucleoSpin RNA Plant May 2014 Rev 09 MACHEREY NAGEL www mn net com RNA isolation from plant Protocol at a glance Rev 09 NucleoSpin RNA Plant 1 Homogenize sample c 100 mg Lyse cells 350 uL RA1 3 5 uL B mercaptoethanol or 350 uL RAP 3 5 uL B mercaptoethanol Mix Filtrate lysate 11 000 x g 1 min Adjust RNA binding conditions 350 uL 70 ethanol Mix Bind R
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17. NucleoSpin® 96 Tissue - MACHEREY

Genomic DNA from tissue User manual NucleoSpin 96 Tissue NucleoSpin 96 Tissue Core Kit May 2014 Rev 07 MACHEREY NAGEL MN www mn net com Genomic DNA from tissue Table of contents 1 Components 1 1 Kit contents 1 2 Reagents to be supplied by user 5 2 Product description 6 2 1 The basic principle 6 2 2 Kit specifications 6 2 3 Required hardware 7 2 4 Accessories supplied for use of the NucleoSpin 96 Tissue Core Kit 8 2 5 Automated processi
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18. NucleoSpin® 8 Blood - MACHEREY

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19. NucleoSpin® DNA FFPE XS - MACHEREY

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20. NucleoSpin® RNA/DNA Buffer Set - MACHEREY

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