| # | Title | Type | Language | |||||||||||
| 1. | NucleoSpin® RNA/Protein - MACHEREY RNA and protein purification User manual NucleoSpin RNA Protein May 2014 Rev 09 MACHEREY NAGEL www mn net com RNA Purification Total RNA and protein isolation Protocol at a glance Rev 09 NucleoSpin RNA Protein 1 Homogenization e a0 tA of sample g 2 Cell lysis 350 uL RP1 3 5 uL B mercapto ethanol 3 Filtration of lysate 11 000 x g E 5 1 min el ag 350 pL ethanol 70 conditions 5 Bind RNA 11 000 x g 30s |
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