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0764 v1.0 Duolink II with Shandon Guidelines - final2
Duolink II GUIDELINES 0764 v1 0 Using Duolink Il with Shandon Sequenza immunostaining system RECOMMENDED MODIFICATIONS TO THE DUOLINK II FLUORESCENCE USER MANUAL DOC NO 0650 OR DUOLINK II BRIGHTFIELD USER MANUAL DOC NO 0656 When using the Duolink Il reagents to stain samples with the Shandon Sequenza immunostaining system a few modifications to the Duolink II protocol optimized for manual staining of samples deposited on glass slides have to be made in order t |
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Duolink In Situ - Fluorescence - Sigma
User Guide Duolink In Situ Fluorescence sigma com SIGMA ALDRICH The protocols in this manual are compatible with all Duolink In Situ PLA probes Duolink In Situ Detection Reagents Green Cat No DUO92014 Orange Cat No DUO92007 Red Cat No DUO92008 and Far Red Cat No DUO92013 Duolink In Situ Fluorescence Table of Contents da JREOQUGHOD eate ett beste mun call 2 Principle of the assay ttn 3 Applications 31 Detect and quantify |
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Duolink ImageTool User Manual - Sigma
Duolink ImageTool USER MANUAL Duolink ImageTool is an image analysis software that objectively quantifies PLA signals and cell nuclei 4 Table of Contents Product Information 5 Image QUISO ont iia 6 Start up Software Installation 7 Fluorescence Analysis cuota ii 8 Brightfeld A alV SIS 12 Support Information Duolink ImageToo Table of Contents Product Information USB FLASH DRIVE The USB flash drive contains the installation file for Duol |
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0656 v3.0 Duolink In Situ Brightfield User Manual
DuolinkelnSitu BIOSCIENCE The protocols in this manual are compatible with all Duolink In Situ PLA probes and Duolink In Situ Detection Reagents Brightfield Art no 92012 Table of content 1 2 9 INTRODUCTION sms satama a eene Ste yes teet PRINCIPLE OF THE ASSAY ette riter tede APPLICATIONS acre pasa 3 1 Detect and quantify protein interactions 3 2 Detect and quantify protein modification 3 3 Detect and quantify protein expression |
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0764 v3.0 Duolink InSitu with Shandon Guidelines
DuolinkeInSitu Using Duolink with Shandon Sequenza immunostaining system RECOMMENDED MODIFICATIONS TO THE DUOLINK IN SITU FLUORESCENCE USER MANUAL DOC NO 0650 OR DUOLINK IN SITU BRIGHTFIELD USER MANUAL DOC NO 0656 When using the Duolink reagents to stain samples with PRE TREATMENT BLOCKING AND PRIMARY the Shandon Sequenza immunostaining system a few ANTIBODIES modifications to the Duolink protocol optimized for manual staining of samples deposited on glass slides |
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0658 v3.0 Duolink In Situ Probemaker Instruction
DuolinkelnSitu BIOSCIENCE Instructions for Duolink In Situ Probemaker PLUS Art no 92009 0020 and Duolink In Situ Probemaker MINUS Art no 92010 0020 Table of content 1 INTRODUCTION 2 APPLICATIONS 2 1 Use antibodies from the same species 2 2 Use antibodies from any species rn 3 ANTIBODY REQUIREMENTS 3 1 Concentration and volume 3 2 Buffer 4 REAGENT PREPARATION AND STORAGE iii 7 5 PROTOCOLS ar |
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0650 v3.1 Duolink In Situ Fluorescence User Manual
DuolinkaeinsSitu BIOSCIENCE The protocols in this manual are compatible with all Duolink In Situ PLA probes Duolink In Situ Detection Reagents Green Art no 92014 Orange Art no 92007 Red Art no 92008 and Far Red Art no 92013 Table of content T INTRODUCTION sad atra E tet 4 2 PRINCIPLE OF THE ASSAY niat te oerte etes 5 8 APPLICATIONS dae ete 6 3 1 Detect and quantify protein interactions 6 3 2 Detect and quantify protein mod |
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Duolink In Situ - Brightfield - Sigma
User Guide Duolink In Situ Brightfeld sigma com SIGMA ALDRICH The protocols in this manual are compatible with all Duolink In Situ PLA probes and Duolink In Situ Detection Reagents Brightfield Cat No DUO92012 Duolink InSitu Table of Contents 1 Introduction N N 2 Principle of the Assay scssssseecsesssssssssesssssssessesssesessesssssstsuussasssassnsssssssseseseesseeseeseeeeeie 3 ADDICAUONS supresa atua iara imensa isca 3 1 Detect and |
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0660 v3.0 Duolink ImageTool Manual - final
0 ImageTool USER MANUAL Duolink ImageTool is an image analysis software that objectively quantifies PLA signals and cell nuclei Table of Contents Product InformatiOn ss cria imc nin 1 06 OM aasta amm eta alin Aaa Start up Software Installation sss Fluorescence Analysis Brightfeld Ari alysiS cerneret as SUP DOME Duolink Image |
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