DNA, RNA, and protein purification - MACHEREY

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1. DNA, RNA, and protein purification - MACHEREY

DNA RNA and protein purification User manual NucleoSpin TriPrep June 2014 Rev 05 MACHEREY NAGEL www mn net com Total DNA RNA and protein isolation Protocol at a glance Rev 05 NucleoSpin TriPrep 1 Homogenize sample e Up to 30 mg 2 Lyse sample T 350 uL RP1 3 5 uL B mercaptoethanol 7 or comparable reducing agent 3 Filtrate lysate El e 1 min 11 000 x g I 4 AdjustDNAand RNA F binding conditions 350 uL ethanol 70
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1. DNA, RNA, and protein purification - MACHEREY

DNA RNA and protein purification User manual NucleoSpin TriPrep June 2014 Rev 05 MACHEREY NAGEL www mn net com Total DNA RNA and protein isolation Protocol at a glance Rev 05 NucleoSpin TriPrep 1 Homogenize sample e Up to 30 mg 2 Lyse sample T 350 uL RP1 3 5 uL B mercaptoethanol 7 or comparable reducing agent 3 Filtrate lysate El e 1 min 11 000 x g I 4 AdjustDNAand RNA F binding conditions 350 uL ethanol 70
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