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1. Total RNA and Protein Isolation

Total RNA and Protein Isolation User manual NucleoSpin RNA Protein November 2005 Rev 01 MACHEREY NAGEL MN Total RNA and Protein Isolation Table of contents 1 Kit contents 2 Product description 2 1 2 2 2 3 2 4 2 5 The basic principle Kit specifications Handling preparation and storage of starting materials Guideline for appropriate sample amount precipitation and resolubilization volume for protein isolation Elution procedures for RNA
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Total RNA and Protein Isolation User Manual NucleoSpin RNA Protein October 2008 Rev 05 MACHEREY NAGEL MN Total RNA and Protein Isolation Protocol at a glance Rev 05 NucleoSpin RNA Protein 1 Homogenization of sample e 30 mg 2 Cell lysis 350 ul RP1 3 5 ul B mercapto ethanol 3 Filtration of S lysate 1 min H 11 000 x g GER AES 350 ul ethanol 70 conditions 5 Bind RNA oS wa 11 000 x g Protein Purification
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