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1. 1.5 Troubleshooting

1 5 Troubleshooting This is a highly reproducible and reliable approach to making large quantities of RNA As long as your DNA is clean phenol extracted and ethanol precipitated miniprep DNA and the enzymes haven t been abused e g left out on the bench etc all should go well When using miniprep DNA remember you must omit the RNAse step or any RNA you synthesize will get chewed up Keep all of your key reagents e g 4NTP cap cap 0 1 M DTT etc in small aliquots frozen at
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