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1. Genomic DNA from Tissue

Genomic DNA from Tissue User Manual NucleoSpin Tissue XS October 2009 Rev 02 MACHEREY NAGEL MN Genomic DNA from Tissue Protocol at a glance Rev 02 1 Prepare sample NucleoSpin Tissue XS Up to 2 5 mg tissue 2 Pre lyse sample 80 ul T1 8 ul Proteinase K 56 C 1 4h 3 Lyse sample 80 ul B3 70 C 5 min 4 Adjust binding conditions 80 ul ethanol 5 Bind DNA Load lysate 1 min 11 000 x g 6 Wash silica 50 ul
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13. Genomic DNA from tissue - MACHEREY

Genomic DNA from tissue User manual NucleoSpin Tissue June 2014 Rev 14 MACHEREY NAGEL www mn net com Genomic DNA from tissue Protocol at a glance Rev 14 1 Prepare sample NucleoSpin Tissue Cut 25 mg into small pieces 2 Pre lyse sample 180 uL T1 25 uL Proteinase K 56 C 1 3 h 3 Lyse sample 200 uL B3 70 C 10 min 4 Adjust DNA binding conditions 210 uL 96 100 ethanol 5 Bind DNA
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