invitrogen pYES2 Cat no V825 20 Version K 5 December 2008 28 0053 Table of Contents Table Of Contento ici ae AAU dl ic end iii Important informa unica tad tardan bin iv NN O NR SS CPTIELPOVE NC E EEEa ania v IntrodU CHO sss sanaan aaea anara aaa aAa ais 1 ONL 814 17 AEREE EEE E E E E E NN NA 1 Cloning into pY ESZ aaaea e ai e RS EA lalola RESE 2 Yeast Transformation aus 2a pnp nRa e R a oa E EN Eaa EA aE EE 5 Expression of Recombinant Protein ccccccccsce
Invitrogen pYES2 1 E and pYC2 E Echo Adapted Expression Vectors For cloning of the gene of interest using the Echo Cloning System and expression in Saccharomyces cerevisiae Catalog nos ET200 XX ET210 XX Version J 29 December 2010 25 0340 ii Table of Contents Kat Contents and Storage 3 0 tt Bro redeo d pa t ed p o A sania iv Accessory Products aec Rau e E Ce o eec LR ree UH E RUE CE lua WE tr dp ges vii INTFOQUCHION WR M 1 jen
invitrogen pYES2 CT pYES3 CT and pYC2 CT Yeast expression vectors with C terminal tags and auxotrophic selection markers Catalog no V8251 20 V8253 20 and V8255 20 Rev date 30 November 2009 Manual part no 25 0304 MAN0000128 ii Table of Contents Kit Contents and Stora gE sr srsti eia iera a AEE RA AN EAE AE ESETE E E ERES SETE E iv Lg lla o 8 oz 4 La y EA e dende 1 TS A A TN 1 Method A 3 Cloning into pYES2 CT pYES3 CT or pYC2 CT eisini eresse i